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pCS2-zfTpo
(Plasmid #168198)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168198 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Total vector size (bp) 4771
  • Vector type
    Mammalian Expression ; Vertebrate expression (zebrafish, chicken, xenopus)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Thrombopoietin
  • Alt name
    tpo
  • Alt name
    thpo
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    682
  • GenBank ID
    EU267076.1
  • Entrez Gene
    thpo (a.k.a. T, tpo)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-zfTpo was a gift from Petr Bartunek (Addgene plasmid # 168198 ; http://n2t.net/addgene:168198 ; RRID:Addgene_168198)

  • For your References section:

    Dissection of vertebrate hematopoiesis using zebrafish thrombopoietin. Svoboda O, Stachura DL, Machonova O, Pajer P, Brynda J, Zon LI, Traver D, Bartunek P. Blood. 2014 Jul 10;124(2):220-8. doi: 10.1182/blood-2014-03-564682. Epub 2014 May 28. 10.1182/blood-2014-03-564682 PubMed 24869937
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