(369) pcDNA3.1(+)-HA-nLaG6-(EAAAK)4-OGA(544-706)
(Plasmid
#168190)
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PurposeHA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGA with residues 544-706. For use with myc-OGA(1-400) split-OGA system.
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Depositing Lab
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Sequence Information
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Sequences (2) — Accept Affinity Reagent Sequence Policy
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Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 168190 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1(+)
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5352
- Total vector size (bp) 6342
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHA-nLag6-(EAAAK)4-OGA(544-706)
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SpeciesH. sapiens (human); Llama Glama
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Insert Size (bp)990
- Promoter T7/CMV
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Tag
/ Fusion Protein
- HA-Tag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Sgfl, PacI (unknown if destroyed)
- 3′ cloning site Sgsl, XbaI (unknown if destroyed)
- 5′ sequencing primer T7 fwd
- 3′ sequencing primer BGH rev (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
(369) pcDNA3.1(+)-HA-nLaG6-(EAAAK)4-OGA(544-706) was a gift from Christina Woo (Addgene plasmid # 168190 ; http://n2t.net/addgene:168190 ; RRID:Addgene_168190) -
For your References section:
O-GlcNAc Engineering on a Target Protein in Cells with Nanobody-OGT and Nanobody-splitOGA. Ramirez DH, Ge Y, Woo CM. Curr Protoc. 2021 May;1(5):e117. doi: 10.1002/cpz1.117. 10.1002/cpz1.117 PubMed 33950562
