XE182 rat Frizzled-1-HA-pCD
(Plasmid #16818)

• Depositing Lab: Randall Moon
• Publication: Xenopus Expression Plasmids (unpublished)

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4095
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: frizzled-1
• Alt name: rfzd1
• Alt name: fzd1
• Species: R. norvegicus (rat)
• Insert Size (bp): 2044
• Entrez Gene: Fzd1
• Tag / Fusion Protein:
  • HA

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: NA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The original rat Frizzled-1 cDNA, tagged with an HA epitope at the Sty1 site of Frizzled1 (third extracellular loop), was cloned into CS2+. The CMV promoter was removed as a Sal-I-HindIII fragment, and the SV40 promoter fragment of the vector pCD-PS (as a Sal-I-SmaI fragment) was cloned in, destroying the HindIII site. As a result, this construct places the cDNA under the control of an SV40 promoter.

Reference: The untagged part of the insert was published by Wang et al., JBC 271: 4468 (1996). The HA-tag insert was made by Jeff Brown, and the remainder of this unpublished construct was generated by Julia Yang-Snyder.