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Addgene

pLVX-dL5**-mCER-TRF1
(Plasmid #168176)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168176 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLVX-IRES-Neo
  • Total vector size (bp) 10904
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRF1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2634
  • Entrez Gene
    TERF1 (a.k.a. PIN2, TRBF1, TRF, TRF1, hTRF1-AS, t-TRF1)
  • Tag / Fusion Protein
    • FAP (dL5**) and mCerulean (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-dL5**-mCER-TRF1 was a gift from Patricia Opresko (Addgene plasmid # 168176 ; http://n2t.net/addgene:168176 ; RRID:Addgene_168176)
  • For your References section:

    Targeted and Persistent 8-Oxoguanine Base Damage at Telomeres Promotes Telomere Loss and Crisis. Fouquerel E, Barnes RP, Uttam S, Watkins SC, Bruchez MP, Opresko PL. Mol Cell. 2019 Jul 11;75(1):117-130.e6. doi: 10.1016/j.molcel.2019.04.024. Epub 2019 May 14. 10.1016/j.molcel.2019.04.024 PubMed 31101499