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p_sc-eVIP
(Plasmid #168174)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168174 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMT
  • Backbone size w/o insert (bp) 8456
  • Total vector size (bp) 9716
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TP53
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1226
  • Mutation
    Wildtype
  • GenBank ID
    NM_000546.5
  • Entrez Gene
    TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
  • Promoter EF-1a
  • Tag / Fusion Protein
    • N/A

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site PmeI (destroyed during cloning)
  • 5′ sequencing primer EF1a-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p_sc-eVIP was a gift from Jesse Boehm & James T. Neal & Aviv Regev (Addgene plasmid # 168174 ; http://n2t.net/addgene:168174 ; RRID:Addgene_168174)

  • For your References section:

    Massively parallel phenotyping of coding variants in cancer with Perturb-seq. Ursu O, Neal JT, Shea E, Thakore PI, Jerby-Arnon L, Nguyen L, Dionne D, Diaz C, Bauman J, Mosaad MM, Fagre C, Lo A, McSharry M, Giacomelli AO, Ly SH, Rozenblatt-Rosen O, Hahn WC, Aguirre AJ, Berger AH, Regev A, Boehm JS. Nat Biotechnol. 2022 Jun;40(6):896-905. doi: 10.1038/s41587-021-01160-7. Epub 2022 Jan 20. 10.1038/s41587-021-01160-7 PubMed 35058622
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