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pJN63 [pmyo-3::BeCyclOp::SL2::mCherry]
(Plasmid #168173)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168173 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCFJ104
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BeCyclOp (codon optimized for C. elegans)
  • Alt name
    BeRhGC, BeGC1, RhoGC
  • Species
    Blastocladiella emersonii

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer gattctttgcttgtcaac
  • 3′ sequencing primer ACTTGTAAGCTTCTAGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJN63 [pmyo-3::BeCyclOp::SL2::mCherry] was a gift from Alexander Gottschalk (Addgene plasmid # 168173 ; http://n2t.net/addgene:168173 ; RRID:Addgene_168173)

  • For your References section:

    Optogenetic manipulation of cGMP in cells and animals by the tightly light-regulated guanylyl-cyclase opsin CyclOp. Gao S, Nagpal J, Schneider MW, Kozjak-Pavlovic V, Nagel G, Gottschalk A. Nat Commun. 2015 Sep 8;6:8046. doi: 10.1038/ncomms9046. 10.1038/ncomms9046 PubMed 26345128
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