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pMS04 [myo-3p::bPGC::SL2::mCherry]
(Plasmid #168172)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168172 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCFJ104
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    bPGC
  • Alt name
    BlgC
  • Species
    Beggiatoa sp.

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer gattctttgcttgtcaac
  • 3′ sequencing primer ACTTGTAAGCTTCTAGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMS04 [myo-3p::bPGC::SL2::mCherry] was a gift from Alexander Gottschalk (Addgene plasmid # 168172 ; http://n2t.net/addgene:168172 ; RRID:Addgene_168172)

  • For your References section:

    Using a Robust and Sensitive GFP-Based cGMP Sensor for Real Time Imaging in Intact Caenorhabditis elegans. Woldemariam S, Nagpal J, Hill T, Li J, Schneider MW, Shankar R, Futey M, Varshney A, Ali N, Mitchell J, Andersen K, Barsi-Rhyne B, Tran A, Costa WS, Krzyzanowski MC, Yu YV, Brueggemann C, Hamilton OS, Ferkey DM, VanHoven M, Sengupta P, Gottschalk A, L'Etoile N. Genetics. 2019 Jul 22. pii: genetics.119.302392. doi: 10.1534/genetics.119.302392. 10.1534/genetics.119.302392 PubMed 31331946
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