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pTH41 [punc-17::YFP-BeCyclOp(A-2x)::SL2::mCherry]
(Plasmid #168170)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168170 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RM#348p
  • Backbone manufacturer
    J. Rand
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YFP-BeCyclOp(A-2x) (codon optimized for C. elegans)
  • Alt name
    YFP-BeRhAC
  • Species
    Blastocladiella emersonii
  • Mutation
    E497K and C566D
  • Tag / Fusion Protein
    • YFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer CCCTCTGAGCCAATC
  • 3′ sequencing primer ACTTGTAAGCTTCTAGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTH41 [punc-17::YFP-BeCyclOp(A-2x)::SL2::mCherry] was a gift from Alexander Gottschalk (Addgene plasmid # 168170 ; http://n2t.net/addgene:168170 ; RRID:Addgene_168170)

  • For your References section:

    Optogenetic tools for manipulation of cyclic nucleotides functionally coupled to cyclic nucleotide-gated channels. Henss T, Nagpal J, Gao S, Scheib U, Pieragnolo A, Hirschhauser A, Schneider-Warme F, Hegemann P, Nagel G, Gottschalk A. Br J Pharmacol. 2021 Mar 18. doi: 10.1111/bph.15445. 10.1111/bph.15445 PubMed 33733470
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