XE241 Rat frizzled-1-GFP-CS2P+
(Plasmid #16817)

• Depositing Lab: Randall Moon
• Publication: Kaykas et al Nat Cell Biol. 2004 Jan . 6(1):52-8.

Backbone

• Vector backbone: pCS2P+
• Backbone size w/o insert (bp): 4095
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: frizzled-1
• Alt name: fz1
• Alt name: frizzled
• Species: R. norvegicus (rat)
• Entrez Gene: Fzd1
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is a fusion protein consisting of rat Frizzled 1 with a carboxy terminal GFP tag.

Reference: A. Kaykas et al., 2004, Nature Cell Biology vol. 6, pp. 52-58.