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Addgene

XE241 Rat frizzled-1-GFP-CS2P+
(Plasmid #16817)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16817 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCS2P+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    frizzled-1
  • Alt name
    fz1
  • Alt name
    frizzled
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Fzd1
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is a fusion protein consisting of rat Frizzled 1 with a carboxy terminal GFP tag.

Reference: A. Kaykas et al., 2004, Nature Cell Biology vol. 6, pp. 52-58.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE241 Rat frizzled-1-GFP-CS2P+ was a gift from Randall Moon (Addgene plasmid # 16817 ; http://n2t.net/addgene:16817 ; RRID:Addgene_16817)
  • For your References section:

    Mutant Frizzled 4 associated with vitreoretinopathy traps wild-type Frizzled in the endoplasmic reticulum by oligomerization. Kaykas A, Yang-Snyder J, Héroux M, Shah KV, Bouvier M, Moon RT. Nat Cell Biol. 2004 Jan . 6(1):52-8. 10.1038/ncb1081 PubMed 14688793