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Addgene

pMS33: pTwinStrep-SUMO-PmcTnsD
(Plasmid #168166)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168166 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ColE1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PmcCAST TnsD
  • Species
    Peltigera membranacea cyanobiont 210A
  • Tag / Fusion Protein
    • 6X His, Twin Strep and SUMO (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMS33: pTwinStrep-SUMO-PmcTnsD was a gift from Feng Zhang (Addgene plasmid # 168166 ; http://n2t.net/addgene:168166 ; RRID:Addgene_168166)

  • For your References section:

    Dual modes of CRISPR-associated transposon homing. Saito M, Ladha A, Strecker J, Faure G, Neumann E, Altae-Tran H, Macrae RK, Zhang F. Cell. 2021 Apr 29;184(9):2441-2453.e18. doi: 10.1016/j.cell.2021.03.006. Epub 2021 Mar 25. 10.1016/j.cell.2021.03.006 PubMed 33770501
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