pMS29: pDonor(PmcCAST)
(Plasmid
#168162)
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PurposePmcCAST donor harboring non-functional cargo.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 168162 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC57
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert namePmcCAST right end (RE)
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SpeciesPeltigera membranacea cyanobiont 210A
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer M13 forward (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namePmcCAST left end (LE)
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SpeciesPeltigera membranacea cyanobiont 210A
Cloning Information for Gene/Insert 2
- Cloning method Gibson Cloning
- 5′ sequencing primer M13 reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMS29: pDonor(PmcCAST) was a gift from Feng Zhang (Addgene plasmid # 168162 ; http://n2t.net/addgene:168162 ; RRID:Addgene_168162) -
For your References section:
Dual modes of CRISPR-associated transposon homing. Saito M, Ladha A, Strecker J, Faure G, Neumann E, Altae-Tran H, Macrae RK, Zhang F. Cell. 2021 Apr 29;184(9):2441-2453.e18. doi: 10.1016/j.cell.2021.03.006. Epub 2021 Mar 25. 10.1016/j.cell.2021.03.006 PubMed 33770501
Map uploaded by the depositor.
