pMS10: pTns(AvCAST)_ΔTnsD
(Plasmid
#168143)
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PurposeInducible expression of AvCAST TnsA, TnsB, TnsC and TniQ proteins.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 168143 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCOLADuet-1
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Backbone manufacturerMillipore Sigma
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameAvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
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SpeciesAnabaena variabilis
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMS10: pTns(AvCAST)_ΔTnsD was a gift from Feng Zhang (Addgene plasmid # 168143 ; http://n2t.net/addgene:168143 ; RRID:Addgene_168143) -
For your References section:
Dual modes of CRISPR-associated transposon homing. Saito M, Ladha A, Strecker J, Faure G, Neumann E, Altae-Tran H, Macrae RK, Zhang F. Cell. 2021 Apr 29;184(9):2441-2453.e18. doi: 10.1016/j.cell.2021.03.006. Epub 2021 Mar 25. 10.1016/j.cell.2021.03.006 PubMed 33770501