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XE42 Rat frizzled-2-CS2+
(Plasmid #16814)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16814 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rat frizzled 2
  • Alt name
    Fzd2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1912
  • Entrez Gene
    Fzd2 (a.k.a. Fz-10, Fzd10)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (destroyed during cloning)
  • 3′ cloning site Xba1 (destroyed during cloning)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert size: ~1912 bp (EcoRI/Xba I both blunted)
Transcription: linearize with Asp 718 and transcribe with SP6.

Plasmid History:
Constructed by Julia Yang-Snyder on the date of 5/19/96.

Original reference: Chan, S.D.H., et al. JBC 267:25202-25207, 1992.

Published reference: Yang-Snyder, J.A. et al. Curr. Biol. 6:1302-1306,
1996.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE42 Rat frizzled-2-CS2+ was a gift from Randall Moon (Addgene plasmid # 16814 ; http://n2t.net/addgene:16814 ; RRID:Addgene_16814)

  • For your References section:

    A frizzled homolog functions in a vertebrate Wnt signaling pathway. Yang-Snyder J, Miller JR, Brown JD, Lai CJ, Moon RT. Curr Biol. 1996 Oct 1. 6(10):1302-6. 10.1016/S0960-9822(02)70716-1 PubMed 8939578
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