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Addgene

pMS4: pHelper(AvCAST)_entry
(Plasmid #168137)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168137 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDFDuet-1
  • Backbone manufacturer
    Millipore Sigma
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    AvCAST minimal CRISPR array
  • Species
    Anabaena variabilis
  • GenBank ID

Gene/Insert 2

  • Gene/Insert name
    AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
  • Species
    Anabaena variabilis

Gene/Insert 3

  • Gene/Insert name
    AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
  • Species
    Anabaena variabilis

Gene/Insert 4

  • Gene/Insert name
    AvTnsD
  • Species
    Anabaena variabilis

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMS4: pHelper(AvCAST)_entry was a gift from Feng Zhang (Addgene plasmid # 168137 ; http://n2t.net/addgene:168137 ; RRID:Addgene_168137)

  • For your References section:

    Dual modes of CRISPR-associated transposon homing. Saito M, Ladha A, Strecker J, Faure G, Neumann E, Altae-Tran H, Macrae RK, Zhang F. Cell. 2021 Apr 29;184(9):2441-2453.e18. doi: 10.1016/j.cell.2021.03.006. Epub 2021 Mar 25. 10.1016/j.cell.2021.03.006 PubMed 33770501
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