pXylP-HygR
(Plasmid
#168131)
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PurposeExpression of HygR from the xylose-inducible xylP promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 168131 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
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Backbone manufacturerNEB
- Backbone size w/o insert (bp) 2686
- Total vector size (bp) 6176
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Vector typeYeast Expression ; Aspergillus Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameXylP-HygR
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Alt namehph
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SpeciesAspergillus fumigatus
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Insert Size (bp)3490
- Promoter xylP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
- 5′ sequencing primer caggaaacagctatgac
- 3′ sequencing primer actggccgtcgttttac (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pXylP-HygR was a gift from Michael Bromley (Addgene plasmid # 168131 ; http://n2t.net/addgene:168131 ; RRID:Addgene_168131)