pGEX6P1-merlin 1-339
(Plasmid
#168128)
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PurposeBacterial expression of human Nf2/merlin 1-339
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 168128 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-6P-1
- Total vector size (bp) 5953
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMerlin
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Alt nameNF2
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SpeciesH. sapiens (human)
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Insert Size (bp)1017
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GenBank ID4471
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Entrez GeneNF2 (a.k.a. ACN, BANF, SCH, SWNV, merlin-1)
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Tag
/ Fusion Protein
- Glutathione S-transferase (GST) (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pGEX 5'
- 3′ sequencing primer pGEX 3' (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX6P1-merlin 1-339 was a gift from Tina Izard (Addgene plasmid # 168128 ; http://n2t.net/addgene:168128 ; RRID:Addgene_168128) -
For your References section:
Conformational flexibility determines the Nf2/merlin tumor suppressor functions. Primi MC, Rangarajan ES, Patil DN, Izard T. Matrix Biol Plus. 2021 Jul 1;12:100074. doi: 10.1016/j.mbplus.2021.100074. eCollection 2021 Dec. 10.1016/j.mbplus.2021.100074 PubMed 34337379
