Gi3-CASE
(Plasmid #168122)

• Purpose: Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).
• Depositing Lab: Gunnar Schulte
• Publication: Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653. doi: 10.1126/scisignal.abf1653. Epub 2021 Sep 7.

Backbone

• Vector backbone: Clontech-style N1
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GNAI2, GNB1, GNG2
• Species: H. sapiens (human)
• Mutation: NLuc is inserted at A114/E115 within GNAI1
• Entrez Gene: GNAI1 (a.k.a. Gi, HG1B, NEDHISB)
• Entrez Gene: GNB1 (a.k.a. HG2A, MDS, MRD42)
• Entrez Gene: GNG2 (a.k.a. HG3F1)
• Tag / Fusion Protein:
  • cpVenus on GNG2 (N terminal on insert)

Cloning Information

• Cloning method: Unknown

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • NanoLuc Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2021.02.05.429900v1 for bioRxiv preprint.