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Addgene

Gi2-CASE
(Plasmid #168121)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168121 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Clontech-style N1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GNAI2, GNB1, GNG2
  • Species
    H. sapiens (human)
  • Mutation
    NLuc is inserted at C112/E115 within GNAI2
  • Entrez Gene
    GNAI2 (a.k.a. GIP, GNAI2B, HG1C, H_LUCA15.1, H_LUCA16.1)
  • Entrez Gene
    GNB1 (a.k.a. HG2A, MDS, MRD42)
  • Entrez Gene
    GNG2 (a.k.a. HG3F1)
  • Tag / Fusion Protein
    • cpVenus on GNG2 (N terminal on insert)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Gi2-CASE was a gift from Gunnar Schulte (Addgene plasmid # 168121 ; http://n2t.net/addgene:168121 ; RRID:Addgene_168121)

  • For your References section:

    Quantitative assessment of constitutive G protein-coupled receptor activity with BRET-based G protein biosensors. Schihada H, Shekhani R, Schulte G. Sci Signal. 2021 Sep 7;14(699):eabf1653. doi: 10.1126/scisignal.abf1653. Epub 2021 Sep 7. 10.1126/scisignal.abf1653 PubMed 34516756
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