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Addgene

pcDNA3.1/SPTBN1-FLT3
(Plasmid #168115)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168115 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 7219
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SPTBN1-FLT3
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_004119.3 NM_178313.3

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHl (not destroyed)
  • 3′ cloning site Xbal (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1/SPTBN1-FLT3 was a gift from Nicholas Cross (Addgene plasmid # 168115 ; http://n2t.net/addgene:168115 ; RRID:Addgene_168115)

  • For your References section:

    A constitutively active SPTBN1-FLT3 fusion in atypical chronic myeloid leukemia is sensitive to tyrosine kinase inhibitors and immunotherapy. Grand FH, Iqbal S, Zhang L, Russell NH, Chase A, Cross NC. Exp Hematol. 2007 Nov;35(11):1723-7. doi: 10.1016/j.exphem.2007.07.002. Epub 2007 Aug 30. 10.1016/j.exphem.2007.07.002 PubMed 17764812
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