Skip to main content
Addgene

pCS2-zfIl34
(Plasmid #168112)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168112 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Total vector size (bp) 4752
  • Vector type
    Mammalian Expression ; Vertebrate expression (zebrafish, chicken, xenopus)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Il34
  • Alt name
    IL-34, interleukin 34
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    657
  • GenBank ID
    NM_001128701
  • Entrez Gene
    il34 (a.k.a. IL-34, si:ch211-239j19.3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer ATTTAGGTGACACTATAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-zfIl34 was a gift from Petr Bartunek (Addgene plasmid # 168112 ; http://n2t.net/addgene:168112 ; RRID:Addgene_168112)

  • For your References section:

    M-CSFR/CSF1R signaling regulates myeloid fates in zebrafish via distinct action of its receptors and ligands. Hason M, Mikulasova T, Machonova O, Pombinho AR, van Ham TJ, Irion U, Nusslein-Volhard C, Bartunek P, Svoboda O. Blood Adv. 2022 Jan 3. pii: 483305. doi: 10.1182/bloodadvances.2021005459. 10.1182/bloodadvances.2021005459 PubMed 34979548
Related items:
Commonly requested with: