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Addgene

pMB1052:miniTol2-U6-(empty)SaCas9_gRNA-CAG-Cre-P2A-NeoR-WPRE
(Plasmid #168108)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168108 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pminiTol2
  • Backbone manufacturer
    Addgene #31829
  • Modifications to backbone
    Disruption of BsaI in AmpR
  • Vector type
    Cre/Lox, CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Heavily modified insert from Addgene #40973 on Addgene #31829 backbone

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMB1052:miniTol2-U6-(empty)SaCas9_gRNA-CAG-Cre-P2A-NeoR-WPRE was a gift from Erich Jarvis (Addgene plasmid # 168108 ; http://n2t.net/addgene:168108 ; RRID:Addgene_168108)

  • For your References section:

    Induction of an immortalized songbird cell line allows for gene characterization and knockout by CRISPR-Cas9. Biegler MT, Fedrigo O, Collier P, Mountcastle J, Haase B, Tilgner HU, Jarvis ED. Sci Rep. 2022 Mar 14;12(1):4369. doi: 10.1038/s41598-022-07434-7. 10.1038/s41598-022-07434-7 PubMed 35288582
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