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pMB952:PB-CAG-PuroR-nGFP-FLEx-SaCas9-P2A-mCherry-WPRE
(Plasmid #168107)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168107 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PBCAG-eGFP
  • Backbone manufacturer
    Addgene #40973
  • Vector type
    Cre/Lox, CRISPR
  • Selectable markers
    Puromycin ; eGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9-P2A-mCherry
  • Alt name
    SaCas9-P2A-mCherry
  • Species
    S.aureus
  • Insert Size (bp)
    4122
  • Promoter CAG
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Puromycin selection disappears following Cre recombination.

Please visit https://www.biorxiv.org/content/10.1101/2021.05.27.445896v1 for BioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMB952:PB-CAG-PuroR-nGFP-FLEx-SaCas9-P2A-mCherry-WPRE was a gift from Erich Jarvis (Addgene plasmid # 168107 ; http://n2t.net/addgene:168107 ; RRID:Addgene_168107)

  • For your References section:

    Induction of an immortalized songbird cell line allows for gene characterization and knockout by CRISPR-Cas9. Biegler MT, Fedrigo O, Collier P, Mountcastle J, Haase B, Tilgner HU, Jarvis ED. Sci Rep. 2022 Mar 14;12(1):4369. doi: 10.1038/s41598-022-07434-7. 10.1038/s41598-022-07434-7 PubMed 35288582
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