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pAc-His-zfCsf1a
(Plasmid #168103)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168103 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAcGP-67B
  • Backbone size w/o insert (bp) 9748
  • Total vector size (bp) 10526
  • Modifications to backbone
    6xHis tag insert
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Csf1a
  • Alt name
    M-CSFa
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    545
  • GenBank ID
    NM_001114480
  • Entrez Gene
    csf1a (a.k.a. csf1-, csf1-1, mcsf, zgc:172186)
  • Tag / Fusion Protein
    • 6x His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CCGGATTATTCATACCGTCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc-His-zfCsf1a was a gift from Petr Bartunek (Addgene plasmid # 168103 ; http://n2t.net/addgene:168103 ; RRID:Addgene_168103)

  • For your References section:

    M-CSFR/CSF1R signaling regulates myeloid fates in zebrafish via distinct action of its receptors and ligands. Hason M, Mikulasova T, Machonova O, Pombinho AR, van Ham TJ, Irion U, Nusslein-Volhard C, Bartunek P, Svoboda O. Blood Adv. 2022 Jan 3. pii: 483305. doi: 10.1182/bloodadvances.2021005459. 10.1182/bloodadvances.2021005459 PubMed 34979548
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