(81) pcDNA3.1-BC2-Nup62-Flag-EPEA
(Plasmid
#168094)
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PurposeNucleoporin Nup62 with N-terminal BC2 nanobody tag and C-terminal Flag and EPEA nanobody tags
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Depositing Lab
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Sequence Information
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Sequences (2) — Accept Affinity Reagent Sequence Policy
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Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 168094 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1(+)
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Backbone manufacturerinvitrogen
- Backbone size w/o insert (bp) 5209
- Total vector size (bp) 7030
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNup62-Ubc-Flag-EPEA
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SpeciesH. sapiens (human)
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Insert Size (bp)1821
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MutationP229A, S283T on Nup62 native sequence
- Promoter T7/CMV
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Tags
/ Fusion Proteins
- BC2-Tag (N terminal on insert)
- Flag-tag (C terminal on insert)
- EPEA-tag (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 fwd
- 3′ sequencing primer BGH rev (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
(81) pcDNA3.1-BC2-Nup62-Flag-EPEA was a gift from Christina Woo (Addgene plasmid # 168094 ; http://n2t.net/addgene:168094 ; RRID:Addgene_168094) -
For your References section:
Target protein deglycosylation in living cells by a nanobody-fused split O-GlcNAcase. Ge Y, Ramirez DH, Yang B, D'Souza AK, Aonbangkhen C, Wong S, Woo CM. Nat Chem Biol. 2021 Mar 8. pii: 10.1038/s41589-021-00757-y. doi: 10.1038/s41589-021-00757-y. 10.1038/s41589-021-00757-y PubMed 33686291
