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pET28a-anticalin (N116amb)-His
(Plasmid #168045)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168045 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5222
  • Total vector size (bp) 5792
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Anticalin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    570
  • Mutation
    Asn 116 was mutated to amber codon (TAG)
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • 6x Histag (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-anticalin (N116amb)-His was a gift from Michael Nash (Addgene plasmid # 168045 ; http://n2t.net/addgene:168045 ; RRID:Addgene_168045)

  • For your References section:

    Mapping Mechanostable Pulling Geometries of a Therapeutic Anticalin/CTLA-4 Protein Complex. Liu Z, Moreira RA, Dujmovic A, Liu H, Yang B, Poma AB, Nash MA. Nano Lett. 2022 Jan 12;22(1):179-187. doi: 10.1021/acs.nanolett.1c03584. Epub 2021 Dec 17. 10.1021/acs.nanolett.1c03584 PubMed 34918516
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