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XE93 mFZ3-CS2+
(Plasmid #16804)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16804 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CS2+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mFZ3-CS2+
  • Alt name
    mfz3
  • Alt name
    fzd3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2300
  • GenBank ID
    U43205
  • Entrez Gene
    Fzd3 (a.k.a. D930050A07Rik, Fz3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 3′ cloning site Xba1 (unknown if destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert: EcoRI to XbaI 2.3 kd clone mFZ3/pRK5 from Nathans lab. Contains full coding (with engineered optimal kozak sequence) sequence of mouse frizzled 3 (accession # U43205)

Plasmid History:
Constructed by: Rebecca Bates/Randall Moon.

Notes for Use:

Ref: Sheldahl et al., Current Biology, 9:695-698, 1999.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE93 mFZ3-CS2+ was a gift from Randall Moon (Addgene plasmid # 16804 ; http://n2t.net/addgene:16804 ; RRID:Addgene_16804)

  • For your References section:

    Protein kinase C is differentially stimulated by Wnt and Frizzled homologs in a G-protein-dependent manner. Sheldahl LC, Park M, Malbon CC, Moon RT. Curr Biol. 1999 Jul 1;9(13):695-8. doi: 10.1016/s0960-9822(99)80310-8. 10.1016/s0960-9822(99)80310-8 PubMed 10395542
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