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Addgene

XE94 mFZ4-CS2+
(Plasmid #16803)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16803 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mFZ4-CS2+
  • Alt name
    mfz4
  • Alt name
    fzd4
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Fzd4 (a.k.a. Fz4)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Cla1 (unknown if destroyed)
  • 3′ cloning site Xba1 (unknown if destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert: The clone mFZ4/pRK5 from Nathans lab (accession # U43317), was used as template to PCR amplify just the coding region 5' (methionine start) site is ClaI, and 3' stop site followed by XbaI.

Constructed by: Rebecca Bates/Randall Moon.

Ref: Sheldahl et al., Current Biology, 9:695-698, 1999.

Addgene results indicate that the first 6aa are correct and there is a large gap from aa 7-143. This is not known to adversely affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE94 mFZ4-CS2+ was a gift from Randall Moon (Addgene plasmid # 16803 ; http://n2t.net/addgene:16803 ; RRID:Addgene_16803)

  • For your References section:

    Protein kinase C is differentially stimulated by Wnt and Frizzled homologs in a G-protein-dependent manner. Sheldahl LC, Park M, Malbon CC, Moon RT. Curr Biol. 1999 Jul 1;9(13):695-8. doi: 10.1016/s0960-9822(99)80310-8. 10.1016/s0960-9822(99)80310-8 PubMed 10395542
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