pET-BoNT/E
(Plasmid
#168020)
-
PurposeExpresses BoNT/E-LC(1-411)-6xHis in bacterial cells
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 168020 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepET (pBR322)
-
Backbone manufacturerDavid Liu Lab
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameBoNT/E-LC(1-411)-6xHis
-
SpeciesSynthetic
-
Tag
/ Fusion Protein
- Hisx6 (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pET-BoNT/E was a gift from David Liu (Addgene plasmid # 168020 ; http://n2t.net/addgene:168020 ; RRID:Addgene_168020) -
For your References section:
Phage-assisted evolution of botulinum neurotoxin proteases with reprogrammed specificity. Blum TR, Liu H, Packer MS, Xiong X, Lee PG, Zhang S, Richter M, Minasov G, Satchell KJF, Dong M, Liu DR. Science. 2021 Feb 19;371(6531):803-810. doi: 10.1126/science.abf5972. 10.1126/science.abf5972 PubMed 33602850