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Addgene

pET-BoNT/E
(Plasmid #168020)

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This material is available to academics and nonprofits only. Availability may be limited outside the U.S. Please log in for more information.
Item Catalog # Description Quantity Price (USD)
Plasmid 168020 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET (pBR322)
  • Backbone manufacturer
    David Liu Lab
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BoNT/E-LC(1-411)-6xHis
  • Species
    Synthetic
  • Tag / Fusion Protein
    • Hisx6 (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-BoNT/E was a gift from David Liu (Addgene plasmid # 168020 ; http://n2t.net/addgene:168020 ; RRID:Addgene_168020)

  • For your References section:

    Phage-assisted evolution of botulinum neurotoxin proteases with reprogrammed specificity. Blum TR, Liu H, Packer MS, Xiong X, Lee PG, Zhang S, Richter M, Minasov G, Satchell KJF, Dong M, Liu DR. Science. 2021 Feb 19;371(6531):803-810. doi: 10.1126/science.abf5972. 10.1126/science.abf5972 PubMed 33602850
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