XE95 mFZ6-CS2+
(Plasmid #16802)

• Depositing Lab: Randall Moon
• Publication: Sheldahl et al Curr Biol. 1999 Jul 1;9(13):695-8. doi: 10.1016/s0960-9822(99)80310-8.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4095
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mFZ6-CS2+
• Alt name: fzd6
• Alt name: fz6
• Species: M. musculus (mouse)
• Entrez Gene: Fzd6 (a.k.a. Fz6)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (unknown if destroyed)
• 3′ cloning site: EcoR1 (unknown if destroyed)
• 5′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert: The clone mFZ4/pRK5 from Nathans lab (accession # U43319), was used as template to PCR amplify just the coding region as an EcoR1 insert. The 3' stop was kept.

Constructed by: Rebecca Bates/Randall Moon.

Notes for Use:

Ref: Sheldahl et al., Current Biology, 9:695-698, 1999.