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Addgene

pLexm-gfp-Pept2
(Plasmid #167988)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167988 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLexM-GFPHis8
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 7600
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PepT2
  • Alt name
    SLC15A2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    2200
  • GenBank ID
    D63149.1
  • Entrez Gene
    Slc15a2
  • Promoter CMV
  • Tag / Fusion Protein
    • cleavable GFP-his8 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer CMV_f
  • 3′ sequencing primer eGFP_N_rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLexm-gfp-Pept2 was a gift from Simon Newstead (Addgene plasmid # 167988 ; http://n2t.net/addgene:167988 ; RRID:Addgene_167988)

  • For your References section:

    Cryo-EM structure of PepT2 reveals structural basis for proton-coupled peptide and prodrug transport in mammals. Parker JL, Deme JC, Wu Z, Kuteyi G, Huo J, Owens RJ, Biggin PC, Lea SM, Newstead S. Sci Adv. 2021 Aug 25;7(35). pii: 7/35/eabh3355. doi: 10.1126/sciadv.abh3355. Print 2021 Aug. 10.1126/sciadv.abh3355 PubMed 34433568
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