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pMRX-IP-EGFP-mouse Plaat3 (WT)
(Plasmid #167987)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167987 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMRX-IPU-EGFP
  • Backbone manufacturer
    Dr.Shoji Yamaoka of Tokyo Medical and Dental University
  • Backbone size w/o insert (bp) 6404
  • Total vector size (bp) 7331
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hrasls3
  • Alt name
    Plaat3
  • Alt name
    Pla2g16
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    489
  • GenBank ID
    NM_001362425.1
  • Entrez Gene
    Plaat3 (a.k.a. AdPLA, HRSL3, Hrasls3, Hrev107, MLP-3, Pla2g16)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    It has the pMX backbone

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMRX-IP-EGFP-mouse Plaat3 (WT) was a gift from Noboru Mizushima (Addgene plasmid # 167987 ; http://n2t.net/addgene:167987 ; RRID:Addgene_167987)

  • For your References section:

    Organelle degradation in the lens by PLAAT phospholipases. Morishita H, Eguchi T, Tsukamoto S, Sakamaki Y, Takahashi S, Saito C, Koyama-Honda I, Mizushima N. Nature. 2021 Apr;592(7855):634-638. doi: 10.1038/s41586-021-03439-w. Epub 2021 Apr 14. 10.1038/s41586-021-03439-w PubMed 33854238
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