pSEVA221TadA*-T7RNAP
(Plasmid
#167980)
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PurposeExpresses fusion protein TadA*-T7RNAP in E. coli
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 167980 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSEVA221
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Backbone manufacturerVictor de Lorenzo
- Backbone size w/o insert (bp) 3719
- Total vector size (bp) 7729
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTadA*-T7RNAP
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SpeciesSynthetic
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Insert Size (bp)4010
- Promoter TetR-Ptet
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Tags
/ Fusion Proteins
- T7 tag (N terminal on insert)
- T7RNAP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSEVA221TadA*-T7RNAP was a gift from Luis Ángel Fernández (Addgene plasmid # 167980 ; http://n2t.net/addgene:167980 ; RRID:Addgene_167980) -
For your References section:
In vivo diversification of target genomic sites using processive base deaminase fusions blocked by dCas9. Alvarez B, Mencia M, de Lorenzo V, Fernandez LA. Nat Commun. 2020 Dec 22;11(1):6436. doi: 10.1038/s41467-020-20230-z. 10.1038/s41467-020-20230-z PubMed 33353963
