pSEP-LAMP1-RFP
(Plasmid #167970)

• Purpose: Expresses the chimeric construct SEP-LAMP1-RFP where superecliptic pHluorin (SEP) is fused to the luminal domain of LAMP1-RFP
• Depositing Lab: Juan Bonifacino
• Publication: Farias et al Proc Natl Acad Sci U S A. 2017 Apr 4;114(14):E2955-E2964. doi: 10.1073/pnas.1616363114. Epub 2017 Mar 20.

Backbone

• Vector backbone: pmRFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4682
• Total vector size (bp): 6620
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LAMP1
• Species: R. norvegicus (rat)
• Insert Size (bp): 1938
• Mutation: D50E
• GenBank ID: NM_012857.2
• Entrez Gene: Lamp1 (a.k.a. LGP120)
• Promoter: CMV
• Tags / Fusion Proteins:
  • SEP (N terminal on insert)
  • mRFP (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV_fwd
• 3′ sequencing primer: SV40_rev

Resource Information

• Supplemental Documents:
  • pSEP-LAMP1-RFP.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry