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Addgene

pBN261
(Plasmid #167966)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167966 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD24
  • Backbone size w/o insert (bp) 4542
  • Total vector size (bp) 6698
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E
  • Species
    Escherichia coli
  • Insert Size (bp)
    2184
  • Mutation
    Y713E, Y715E, Y717E
  • Promoter araBAD
  • Tag / Fusion Protein
    • hexa-histidine tag (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBN261 was a gift from James Naismith (Addgene plasmid # 167966 ; http://n2t.net/addgene:167966 ; RRID:Addgene_167966)

  • For your References section:

    The molecular basis of regulation of bacterial capsule assembly by Wzc. Yang Y, Liu J, Clarke BR, Seidel L, Bolla JR, Ward PN, Zhang P, Robinson CV, Whitfield C, Naismith JH. Nat Commun. 2021 Jul 16;12(1):4349. doi: 10.1038/s41467-021-24652-1. 10.1038/s41467-021-24652-1 PubMed 34272394
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