XE129 beta2AR Fz1-pcDNA3.1
(Plasmid
#16795)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16795 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCDNA3.1
- Backbone size w/o insert (bp) 5428
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Vector typeXenopus Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Chimera of beta 2 adrenergic receptor (extracellular and.
transmembrane domains) and rat frizzled-1 (intracellular). Activated by isoproterenol and inhibited by propranolol.
Ref: X. Lui et al., PNAS, 1999, 96:14383-14388 and M. Kuehl et al.,
2000, JBC 275:12701-12711.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
XE129 beta2AR Fz1-pcDNA3.1 was a gift from Randall Moon (Addgene plasmid # 16795 ; http://n2t.net/addgene:16795 ; RRID:Addgene_16795) -
For your References section:
Activation of a frizzled-2/beta-adrenergic receptor chimera promotes Wnt signaling and differentiation of mouse F9 teratocarcinoma cells via Galphao and Galphat. Liu X, Liu T, Slusarski DC, Yang-Snyder J, Malbon CC, Moon RT, Wang H. Proc Natl Acad Sci U S A. 1999 Dec 7;96(25):14383-8. doi: 10.1073/pnas.96.25.14383. 10.1073/pnas.96.25.14383 PubMed 10588714