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Addgene

pBAD-eLACCO1.1
(Plasmid #167944)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167944 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD
  • Backbone manufacturer
    Thermo Fisher Scientific
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 5834
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eLACCO1.1
  • Species
    Synthetic
  • Insert Size (bp)
    1997
  • Promoter araBAD

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pBAD forward
  • 3′ sequencing primer pBAD reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD-eLACCO1.1 was a gift from Robert Campbell (Addgene plasmid # 167944 ; http://n2t.net/addgene:167944 ; RRID:Addgene_167944)

  • For your References section:

    A genetically encoded fluorescent biosensor for extracellular L-lactate. Nasu Y, Murphy-Royal C, Wen Y, Haidey JN, Molina RS, Aggarwal A, Zhang S, Kamijo Y, Paquet ME, Podgorski K, Drobizhev M, Bains JS, Lemieux MJ, Gordon GR, Campbell RE. Nat Commun. 2021 Dec 6;12(1):7058. doi: 10.1038/s41467-021-27332-2. 10.1038/s41467-021-27332-2 PubMed 34873165
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