Skip to main content
Addgene

pLenti-tetON-dxCas9(3.7)-VPR-EF1a-TagRFP-2A-tet3G
(Plasmid #167937)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167937 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pHAGE
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dxCas9(3.7)-VPR
  • Species
    Synthetic
  • Promoter tetON

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer gacagatccattcgattagtg
  • 3′ sequencing primer GCTCAAGGGGCTTCATGATG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

dxCas9(3.7)-VPR is cloned from dxCas(3.7)-VPR (Addgene Plasmid #108383).

Please visit https://www.biorxiv.org/content/10.1101/2020.05.04.076067v2 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-tetON-dxCas9(3.7)-VPR-EF1a-TagRFP-2A-tet3G was a gift from Emma Rawlins (Addgene plasmid # 167937 ; http://n2t.net/addgene:167937 ; RRID:Addgene_167937)

  • For your References section:

    A functional genetic toolbox for human tissue-derived organoids. Sun D, Evans L, Perrone F, Sokleva V, Lim K, Rezakhani S, Lutolf M, Zilbauer M, Rawlins EL. Elife. 2021 Oct 6;10. pii: 67886. doi: 10.7554/eLife.67886. 10.7554/eLife.67886 PubMed 34612202
Related items:
Commonly requested with: