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Purpose(Empty Backbone) All-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 167934 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonelentiSAMv2
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Backbone manufacturerFeng Zhang (Addgene Plasmid #75112)
- Backbone size (bp) 13500
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Modifications to backboneInserting the P2A-MS2-p65-HSF1 fragment from the lentiMPHv2 plasmid (Addgene Plasmid #89308) at the BsrGI site.
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Vector typeMammalian Expression, Lentiviral, CRISPR
- Promoter U6, EF1a
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LentiSAMPHv2 was a gift from Ronald Germain (Addgene plasmid # 167934 ; http://n2t.net/addgene:167934 ; RRID:Addgene_167934) -
For your References section:
Efficient Immune Cell Genome Engineering with Enhanced CRISPR Editing Tools. Chan W, Gottschalk RA, Yao Y, Pomerantz JL, Germain RN. Immunohorizons. 2021 Feb 23;5(2):117-132. doi: 10.4049/immunohorizons.2000082. 10.4049/immunohorizons.2000082 PubMed 33622708
