LentiGuide-TagBFP-2A-BSD
(Plasmid #167929)

• Purpose: (Empty Backbone) Lentiviral gRNA expression with TagBFP fluorescence and blasticidin selection marker.
• Depositing Lab: Ronald Germain
• Publication: Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132. doi: 10.4049/immunohorizons.2000082.

Backbone

• Vector backbone: lentiCRISPR v2
• Backbone manufacturer: Feng Zhang (Addgene Plasmid #52961)
• Backbone size (bp): 14873
• Modifications to backbone: Replacing the Cas9-P2A-PuroR fragment in the backbone with the TagBFP-P2A-BSD fragment.
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Promoter: U6, EF1a
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry