NAX_CAGGS_CAS9_m4_KRAB_KanR neo
(Plasmid
#167877)
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PurposePiggyBac compatible plasmid expressing dCas9-KRAB
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 167877 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepB-CAGGS
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Vector typeCRISPR
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedCas9-KRAB
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SpeciesSynthetic
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MutationD10A, D839A, H840A, N863A
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NAX_CAGGS_CAS9_m4_KRAB_KanR neo was a gift from Alejandro Chavez & Nan Cher Yeo (Addgene plasmid # 167877 ; http://n2t.net/addgene:167877 ; RRID:Addgene_167877) -
For your References section:
Generation and application of a versatile CRISPR toolkit for mammalian cell engineering. Nghi Dang , Alissa Lance-Byrne, Angela Tung, Xiaoge Guo, Ryan J Cecchi, Joanna Buchthal, Alejandro Chavez, Nan Cher Yeo. Synthetic Biology, Volume 6, Issue 1, 2021 10.1093/synbio/ysab033