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Addgene

NAX_CAGGS_CAS9_m4_KRAB_EBFP2
(Plasmid #167873)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167873 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pB-CAGGS
  • Vector type
    CRISPR
  • Selectable markers
    EBFP2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9-KRAB
  • Species
    Synthetic
  • Mutation
    D10A, D839A, H840A, N863A

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NAX_CAGGS_CAS9_m4_KRAB_EBFP2 was a gift from Alejandro Chavez & Nan Cher Yeo (Addgene plasmid # 167873 ; http://n2t.net/addgene:167873 ; RRID:Addgene_167873)

  • For your References section:

    Generation and application of a versatile CRISPR toolkit for mammalian cell engineering. Nghi Dang , Alissa Lance-Byrne, Angela Tung, Xiaoge Guo, Ryan J Cecchi, Joanna Buchthal, Alejandro Chavez, Nan Cher Yeo. Synthetic Biology, Volume 6, Issue 1, 2021 10.1093/synbio/ysab033
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