XE122 XDsh delta DIX-GFP-CS2+
(Plasmid #16787)

• Depositing Lab: Randall Moon
• Publication: Miller et al J Cell Biol. 1999 Jul 26;146(2):427-37. doi: 10.1083/jcb.146.2.427.

Backbone

• Vector backbone: PCS2+
• Backbone size w/o insert (bp): 4095
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Xdsh-delta DIX
• Alt name: dsh
• Species: X. laevis (frog)
• Mutation: deletion of aa 1-159
• Entrez Gene: dvl2.L (a.k.a. XELAEV_18019661mg, dishevelled, dsh, dvl, dvl2, xdd1, xdsh)
• Tag / Fusion Protein:
  • gfp

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

XDsh-GFP is from J. Yang-Snyder et al. (Curr. Biol., 1996).
A deletion of amino acids 1-159 was conducted and a new start met was introduced.

Linearize with Not1 and transcribe with SP6.

Ref: J.R. Miller et al., (1999), J. Cell Biology 146:427-437.