XE123 XDsh delta PDZ-GFP- CS2+
(Plasmid
#16786)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16786 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4095
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Vector typeXenopus Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameXdsh-delta-PDZ
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Alt namedsh
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SpeciesX. laevis (frog)
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Mutationcontains Dsh amino acids 1-267 and 381-736 (deleting 268-380)
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Entrez Genedvl2.L (a.k.a. XELAEV_18019661mg, dishevelled, dsh, dvl, dvl2, xdd1, xdsh)
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Tag
/ Fusion Protein
- gfp
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer Sp6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GFP-tagged XDsh of Yang-Snyder et al. (Curr. Biology, 1996)
was engineered to now contain Dsh amino acids 1-267 and 381-736 (deleting 268-380).
Linearize with Not1 and transcribe with SP6.
Ref: J.R. Miller et al., (1999), J. Cell Biology 146:427-437.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
XE123 XDsh delta PDZ-GFP- CS2+ was a gift from Randall Moon (Addgene plasmid # 16786 ; http://n2t.net/addgene:16786 ; RRID:Addgene_16786) -
For your References section:
Establishment of the dorsal-ventral axis in Xenopus embryos coincides with the dorsal enrichment of dishevelled that is dependent on cortical rotation. Miller JR, Rowning BA, Larabell CA, Yang-Snyder JA, Bates RL, Moon RT. J Cell Biol. 1999 Jul 26;146(2):427-37. doi: 10.1083/jcb.146.2.427. 10.1083/jcb.146.2.427 PubMed 10427095