p28BIOHTEV-LIC
(Plasmid
#167852)
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Purpose(Empty Backbone) Bacterial expression vector with N-terminal AviTag and C-terminal 6X-his tag for protein expression and purification
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 167852 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
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Tags
/ Fusion Proteins
- AviTag (N terminal on backbone)
- 6xHis (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
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Tags
/ Fusion Proteins
- AviTag (N terminal on backbone)
- 6xHis (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
More information about this cloning vector can be found at https://www.thesgc.org/sites/default/files/toronto_vectors/p28BIOHTEV-LIC.pdf
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p28BIOHTEV-LIC was a gift from Cheryl Arrowsmith (Addgene plasmid # 167852 ; http://n2t.net/addgene:167852 ; RRID:Addgene_167852)
