pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2
(Plasmid
#167849)
-
PurposeBacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 167849 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
* Log in to view industry pricing.
Backbone
-
Vector backbonepET28-MHL
-
Backbone manufacturerSGC (Addgene Plasmid #26096)
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert namensp10, nsp16
-
SpeciesSARS-CoV-2
-
Insert Size (bp)1479
- Promoter T7
-
Tag
/ Fusion Protein
- 6xHis-TEV (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
SGC clone sample ID: nsp16_SARS2-nsp10_SARS2-MVC019-A07:C245477
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2 was a gift from Cheryl Arrowsmith (Addgene plasmid # 167849 ; http://n2t.net/addgene:167849 ; RRID:Addgene_167849) -
For your References section:
A High-Throughput RNA Displacement Assay for Screening SARS-CoV-2 nsp10-nsp16 Complex toward Developing Therapeutics for COVID-19. Perveen S, Khalili Yazdi A, Devkota K, Li F, Ghiabi P, Hajian T, Loppnau P, Bolotokova A, Vedadi M. SLAS Discov. 2021 Jan 10:2472555220985040. doi: 10.1177/2472555220985040. 10.1177/2472555220985040 PubMed 33423577
