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p28BIOHTEV-LIC: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2
(Plasmid #167848)

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Item Catalog # Description Quantity Price (USD)
Plasmid 167848 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    p28BIOHTEV-LIC
  • Backbone manufacturer
    SGC Toronto
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    nsp10, nsp16
  • Species
    SARS-CoV-2
  • Insert Size (bp)
    1479
  • Promoter T7
  • Tags / Fusion Proteins
    • AviTag (N terminal on backbone)
    • 6xHis (C terminal on backbone)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SGC clone sample ID: nsp16_SARS2-nsp10_SARS-MVC019-A11:C245481

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p28BIOHTEV-LIC: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2 was a gift from Cheryl Arrowsmith (Addgene plasmid # 167848 ; http://n2t.net/addgene:167848 ; RRID:Addgene_167848)

  • For your References section:

    A High-Throughput RNA Displacement Assay for Screening SARS-CoV-2 nsp10-nsp16 Complex toward Developing Therapeutics for COVID-19. Perveen S, Khalili Yazdi A, Devkota K, Li F, Ghiabi P, Hajian T, Loppnau P, Bolotokova A, Vedadi M. SLAS Discov. 2021 Jan 10:2472555220985040. doi: 10.1177/2472555220985040. 10.1177/2472555220985040 PubMed 33423577
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