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Addgene

WDR55
(Plasmid #167844)

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Item Catalog # Description Quantity Price (USD)
Plasmid 167844 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pFBOH-MHL
  • Backbone manufacturer
    SGC (Addgene Plasmid #62304)
  • Vector type
    Baculovirus expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    WDR55
  • Alt name
    PBD ID: 7KQQ
  • Alt name
    SGC clone sample ID: PBC015-H03:C242173
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    942
  • Entrez Gene
    WDR55 (a.k.a. FLJ20195, FLJ21702)
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • 6xHis-TEV (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer pFBOH-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3')
  • 3′ sequencing primer pFBOH-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3')
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    WDR55 was a gift from Cheryl Arrowsmith (Addgene plasmid # 167844 ; http://n2t.net/addgene:167844 ; RRID:Addgene_167844)
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