pMAL659
(Plasmid
#167838)
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PurposeEncodes OptoLAC2B circuit
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 167838 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namelacI
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SpeciesEscherichia coli
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Insert Size (bp)1080
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Tag
/ Fusion Protein
- ssrA (AAV) (C terminal on insert)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byAddgene #43795 and #43796
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMAL659 was a gift from Jose Avalos (Addgene plasmid # 167838 ; http://n2t.net/addgene:167838 ; RRID:Addgene_167838) -
For your References section:
Optogenetic control of the lac operon for bacterial chemical and protein production. Lalwani MA, Ip SS, Carrasco-Lopez C, Day C, Zhao EM, Kawabe H, Avalos JL. Nat Chem Biol. 2021 Jan;17(1):71-79. doi: 10.1038/s41589-020-0639-1. Epub 2020 Sep 7. 10.1038/s41589-020-0639-1 PubMed 32895498