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Addgene

pMAL294
(Plasmid #167834)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167834 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lacI
  • Species
    Escherichia coli
  • Insert Size (bp)
    1080
  • Tag / Fusion Protein
    • ssrA (LAA) (C terminal on insert)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Addgene #43795 and #43796

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMAL294 was a gift from Jose Avalos (Addgene plasmid # 167834 ; http://n2t.net/addgene:167834 ; RRID:Addgene_167834)

  • For your References section:

    Optogenetic control of the lac operon for bacterial chemical and protein production. Lalwani MA, Ip SS, Carrasco-Lopez C, Day C, Zhao EM, Kawabe H, Avalos JL. Nat Chem Biol. 2021 Jan;17(1):71-79. doi: 10.1038/s41589-020-0639-1. Epub 2020 Sep 7. 10.1038/s41589-020-0639-1 PubMed 32895498
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