Skip to main content
Addgene

XE160 XDshHA-pt mutant PDZ-CS2+
(Plasmid #16780)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16780 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Dishevelled
  • Alt name
    Dsh
  • Species
    X. laevis (frog)
  • Mutation
    point mutation in the binding pocket of the PDZ binding domain
  • Entrez Gene
    dvl2.L (a.k.a. XELAEV_18019661mg, dishevelled, dsh, dvl, dvl2, xdd1, xdsh)
  • Tag / Fusion Protein
    • HA

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Description: HA tagged Xenopus Dishevelled with a point mutation in the binding pocket of the PDZ binding domain, that blocks binding to Dapper.

Notes for Use: Linearize with either Not1 or Asp718.

Reference: B. Cheyette et al. (2002). Developmental Cell 2: 449-461.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE160 XDshHA-pt mutant PDZ-CS2+ was a gift from Randall Moon (Addgene plasmid # 16780 ; http://n2t.net/addgene:16780 ; RRID:Addgene_16780)

  • For your References section:

    Dapper, a Dishevelled-associated antagonist of beta-catenin and JNK signaling, is required for notochord formation. Cheyette BN, Waxman JS, Miller JR, Takemaru K, Sheldahl LC, Khlebtsova N, Fox EP, Earnest T, Moon RT. Dev Cell. 2002 Apr. 2(4):449-61. 10.1016/S1534-5807(02)00140-5 PubMed 11970895
Related items:
Commonly requested with: